Microbiological Studies
Microbiology laboratory at SRI undertakes quality inspection and
certification for industries associated with raw and processed food, drugs,
pharmaceuticals, cosmetics and water. The lab is well equipped, with state
of the art facilities and experienced scientists. The lab provides the
following services.
Thrust Areas
- Product Quality Certification
- Projects
- Consultancy
- Training
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Product Quality Inspection And Certification
- Detection Of Pathogens
- Enumeration of Spoilage Micro-Flora
- Residual Anti-Microbial Substances
- Bacterial Enterotoxins
- Mycotoxins
- Disinfection Efficacy
- Preservative Efficacy
- Potency of Antibiotics And Vitamins
- Sterility Testing And Bio-Burden
- Microbiological Monitoring of Indoor Air
- Anti Microbial efficacy of nano technology based devices/filters.
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Detection Of Pathogens
Name Of The Pathogens
- Escherichia coli
- Escherichia coli O157
- Vibrio cholera
- Vibrio parahaemolyticus
- Pathogenic streptococci
- Staphylococcus aureus
- Pseudomonas aeruginosa
- Legionella
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- Campylobacter jejuni
- Clostridium perfringens
- Yersinia enterocolitica
- Bacillus cereus
- Salmonella
- Shigella
- Listeria monocytogenes
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Protocols /Techniques Followed
- FDA-BAM
- APHA
- BIS
- ASTM
- AOAC
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- ELISA
- Immuno Enrichment
- Immuno Capture
- Conventional Methods
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Enumeration Of Spoilage Microbes
Micro-organisms are ubiquitous in nature. Quality and shelf life of any
product depends on presence and nature of micro-organisms contained in it.
Under favourable conditions these micro organisms proliferate and result in
loss of product quality. Microbiological monitoring is an essential part of
quality control. SRI plays an important role in testing and certification.
Test Parameters
- Standard Plate Count
- Coliform / Faecal Coliforms
- Psychrophilic and Thermophilic
- Aerobic and an-aerobic spore formers
- Rope Spore Count
- Anaerobic Bacteria
- Sulphite Reducing Bacteria
- Sulphur and Iron Bacteria
- Yeast and Moulds
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Residual Anti-Microbial Substances
Food-producing animals are given antimicrobial drugs for prevention of
disease and for their well being. The residues of these antimicrobial drugs
remain in and on the body of the animals.
Following anti-microbial residues are being tested at ppb levels using
ELISA techniques.
- Tetracyclines
- Sulfonamides
- Streptomycin
- Chloramphenicol
- Gentamycin
- Neomycin
- Nitrofurans etc .
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Mycotoxins
Mycotoxins are carcinogenic, highly toxic metabolites of fungi like
Aspergillus, Fusarium and Penicillium. They are toxic even in trace
quantities.
- Aflatoxins -Aflatoxin B1, B2, G1, G2, M1
- Fumonisin
- Deoxynivalenol (Vomitoxin)
- Ochratoxin A
- Zearalenone
- T-2 toxin etc.
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SRI undertakes the analysis of above toxic compounds/metabolites using
sensitive techniques such as HPLC, HPTLC,LCMS-MS & ELISA at sub ppb
levels.
Bacterial Enterotoxins
Bacteria produces Enterotoxins as a part of their metabolic activities.
Some of them are heat labile while others are heat stable in nature. Even
severe food processing treatments can not eradicate these toxicants and may
result in food intoxication.
SRI undertakes testing of the following Enterotoxins in food and food
products.
- Bacillus cereus Diarrhoeal enterotoxins
- Staphylococcal enterotoxins
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Sterility Testing
Sterility is a mandatory requirement for all injectables, ophthalmic
preparations and surgical devices. Sterility test is conducted in accordance
with various pharmacopoeia viz.. IP, USP, BP etc. Following products are
being tested.
- Parenteral Preparations (Injectables)
- Non-parenteral Preparations (Ophthalmic)
- Mechanical Contraceptives
- Surgical dressings and Devices
- Vaccines
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Bioburden Estimation
Terminally sterilized products are assessed for their microbial loads prior
to sterilization. This helps in standardization of sterilization loads. SRI
also undertakes method validation studies for bio-burden estimation in
surgical devices.
Monitoring of Indoor Air
SRI carries out air-monitoring studies for presence of microbes. Services
are provided in the following areas.
- Storage Godowns
- Hotels/Restaurants
- Food Processing/Packaging Areas
- Clean Rooms/Clean Air Devices
- Operation Theatres and Intensive care units in hospitals
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Anti Bacterial Efficacy of
Nanotechnology based devices/filters
Nanotechnology based devices are the ones which show efficacy even while
working on nano scale(one billionth). Various devices are based on this
technology such as washing machines, refrigerators, AC etc. Similarly
filters are used for water purification and are based on various techniques
like UV-disinfection, ozonization, ion exchange and membrane filtration.
Filters are checked for anti bacterial efficacy against a number of
challenge organisms like E.coli, Salmonella, Shigella, Vibrio,
Staphylococcus, Pseudomonas and Micrococcus luteus.
Preservative Efficacy
Nanotechnology In pharmaceutical, herbal and cosmetic formulations, various
anti-microbial substances are added to arrest microbial activity and extend
shelf life of the products. The effectiveness of these substances are
evaluated as per USP protocols. Selection of challenge micro-organisms
include gram positive and gram negative bacteria, spore formers, yeast and
fungi.
Disinfection Efficacy
The Phenolic and non-phenolic type disinfectants are being tested for their
germicidal value as per National and International protocols.